One of the continuing, persistent challenges confronting tissue engineering is the lack of intrinsic microvessels for the transportation of nutrients and metabolites. An artificial microvascular system can be a feasible solution to this problem. In this study, the femtosecond laser ablation technique was implemented for the fabrication of pillared microvessel scaffolds in PLGA. This novel scaffold enable the conventional cell seeding process to be implemented and the progress of cell growth to be observed in vitro by an optical microscopy. Hence, the milky and completely opaque problems of the conventional PLGA scaffold after cell seeding can be resolved. Currently, PLGA microvessel scaffolds consisting of 30μm×200μm pillared branches have been produced. Cell cultural results of BECs demonstrate that cells can well adhere and grow surrounding each branch of the proposed pillared microvessel networks. The promising results reveal that an artificial microvessel networks for tissue engineering can be completely realized.
- Design Engineering Division and Computers and Information in Engineering Division
Fabrication of Pillared PLGA Microvessel Scaffold Using Femtosecond Laser Ablation
Wang, H, Cheng, C, Li, C, & Wang, G. "Fabrication of Pillared PLGA Microvessel Scaffold Using Femtosecond Laser Ablation." Proceedings of the ASME 2011 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. Volume 7: 5th International Conference on Micro- and Nanosystems; 8th International Conference on Design and Design Education; 21st Reliability, Stress Analysis, and Failure Prevention Conference. Washington, DC, USA. August 28–31, 2011. pp. 33-39. ASME. https://doi.org/10.1115/DETC2011-47532
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