The establishment of the sterilization technology in non-heating environment is requested in the food industry and a lot of other fields. The existing sterilization technology using the high pressure is mainly liberating rapid pressure and a lot of loadings. In this research, the sterilization by a high pressure was tried by using the underwater shock wave caused by explosive. The detonating fuse was detonated with the percussion cap, and the shock wave was generated. Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Saccharomyces cerevisiae were used. The underwater shock wave barely causes heat. An excellent bactericidal effect of 100% was obtained in the maximum in the experiment that used S. cerevisiae.

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