Injecting low-salinity water has proved to be an efficient displacement process in oil reservoirs, owing to its ability to modify the properties at the fluid-rock and fluid-fluid interfaces in favor of mobilizing more oil. In this regard, reduction of interfacial tension (IFT) between oil and water is one of the key controlling parameters. It is suspected that the asphaltene constituents of the oil and type of water ions are responsible for such a reduction in IFT. In this study, systematic experimental investigations were carried out to scrutinize the influence of brine salinity, asphaltene concentration, and temperature on IFT. Single and multi-component brines, which in particular compose of NaCl, CaCl2, and MgCl2 salts, and two synthetic oils with 1 and 10 wt% asphaltene content were used at temperatures ranging from 25 to 80 °C. The results showed that the presence of salt in the solution can alter the distribution of polar components at the oil-brine interface due to the electrostatic effects, which in turn would change IFT of the system. IFT also decreased when temperature increased from 25 to 80 °C; however, the level of changes was strongly depended on the brine type, salinity level, and asphaltene content. The results also demonstrated that the crude oil with the higher asphaltene concentration experiences higher IFT reduction when is contacted with the low-salinity water. The new findings from this study will improve the understanding of the underlying mechanisms for low salinity water flooding in oil reservoirs.