The primary cause of mechanical-related fish injury and mortality in turbomachinery is blade strike. Fish contained in the flow may strike with the rotor blades and the fixed diffuser vanes, the latter being a non-negligible factor causing fish damage in the pump system. In this study, an experiment-based correlation of fish mutilation ratio acts as critical strike velocity. The relation between strike damage in a vaned diffuser and the theoretical pump head is presented as a function of specific speed. As an example, a vaned diffuser is designed for a single-bladed, mixed-flow impeller with the purpose of improving fish friendliness. This pump can be scaled to operate with a head up to 14 m at peak efficiency, without fish damage in the diffuser. Subsequently, experiments are conducted to show the retained pump performance as well as the great improvement of fish friendliness.